Colorimetric measurement of dermatan sulphate
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چکیده
منابع مشابه
Structure and properties of dermatan sulphate proteoglycans.
Introduction Fibrous connective tissues like sclera, skin, tendon and cornea have a high content of collagen but only small amounts of proteoglycans and structural glycoproteins. The specific blend of collagen and proteoglycan types determines the properties of the tissue. The proteoglycans are characterized by having a central protein core substituted by one or several glycosaminoglycan chains...
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Dermatan sulphate (DS) is a glycosaminoglycan which selectively catalyzes the inactivation of thrombin by Heparin Cofactor II without interacting with Antithrombin III. DS does not interact with other coagulation factors and, unlike heparin, is able to inactivate thrombin bound to fibrin or to the surface of an injured vessel. Efficacy and safety of DS have been validated in studies on thrombop...
متن کاملA method for the sequence analysis of dermatan sulphate.
We are attempting to develop methods for the sequencing of glycosaminoglycans from their reducing end. Here we describe a procedure for the analysis of dermatan sulphate from pig skin. The glycosaminoglycan is released from its parent proteoglycan by exhaustive proteolysis by using both endo- and exo-peptidases. The amino group of the residual serine residue is conjugated with a p-hydroxyphenyl...
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1. Proteoglycans were extracted from sclera with 4 M-guanidine hydrochloride in the presence of proteinase inhibitors and purified by ion-exchange chromatography and density-gradient centrifugation. 2. The entire proteoglycan pool was characterized by compositional analyses and by specific chemical (periodate oxidation) and enzymic (chondroitinases) degradations. The glycan moieties of the mole...
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Ledbetter, S. R., Tyree, B.. Hashell. J. R. & Horrigan. E. A. ( 1985) J . Biol. C'hrm. 260,8106-8 I 13 Hassell, J. R., Noonan, D. M., Ledbetter, S. R. & Laurie. G. W. (1986) C'ihri Found. Symp. 124,204-222 Noonan. D. M.. Horrigan. E. A.. Ledbetter. S. R.. Vogeli. G., Sasaki. M.. Yamada. Y. & Hassell. J . R. ( 1988) J . Riol. ('hem. 263, 16379-16387 Paulsson. M.. Yurchenko. P. D.. Ruben. G. C.. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1240549